The Neurobiology of Violence

In the United States, homicide accounts for approximately 20,000 deaths annually.¹ A victimization survey estimated that approximately 4.2 violent crimes (assaults, robberies, and rapes) occur annually for each 100 persons older than 12 years.¹ Another survey of the general adult population found a 3.7% annual rate of self-reported violent behavior against other persons.² Thus, violent crime and violent behavior in general cause a major public health problem.

Societal and cultural factors play an important role in the development of violent behavior. However, these environmental factors and their fluctuations elicit different responses in different people. Violent behavior develops as a result of complex interactions between neurobiological and environmental factors. This review will focus primarily on neurobiology. Some of the neurobiological mechanisms of violent behavior are similar or identical to those that operate in suicidal behavior.³

Substance use, personality disorders, major mental disorders, head injuries, and other diagnosable disorders contribute to the level of community violence. However, recent evidence points to neurobiological mechanisms that affect the development and the control of violent behavior but that are not clearly linked to the existing diagnostic categories. These mechanisms involve neurotransmitters that are in part under genetic control; the details are emerging in current research efforts. The main purpose of this article is to update a previous review,⁴ stressing the neurobiological commonalities between aggressive and suicidal behavior.

DEFINITION AND SUBTYPES OF VIOLENT BEHAVIOR

For the purpose of this review, violent behavior is defined as overt and intentional physically aggressive behavior against another person. Examples include beating, kicking, choking, pushing, grabbing, throwing objects, using a weapon, threatening to use a weapon, and forcing sex. The definition does not include aggression against self. Violent crimes include murder, robbery, assault, and rape. In this review, I will not deal with organized state violence or ethnic warfare.

Violent behavior is heterogeneous in its origins and manifestations. Nevertheless, most violent acts can be classified as impulsive or premeditated, and many perpetrators can be classified as committing predominantly impulsive or predominantly premeditated violent acts. There is increasing evidence for the validity of this distinction. Phenytoin reduced impulsive, but not premeditated, violent acts among prisoners.⁵ Impulsive violent offenders had lower verbal skills than those committing premeditated violent acts.⁶ Persons who committed impulsive murders differed from those who committed premeditated ones in their pattern of brain glucose utilization.⁷ Premeditated violent acts may be either predatory (committed for one's own gain; for example, a robbery) or pathological (committed, for example, by mentally ill people acting on their delusions or hallucinations). The mentally ill, however, may also commit predatory or impulsive violent acts.

CLINICAL CORRELATES OF VIOLENT BEHAVIOR

Substance use disorders (alcohol and drugs) play a major role in violent and suicidal behavior. In the United States, 34% of the risk of violence self-reported by community residents is attributable to substance abuse.² Urine tests were positive for illicit drugs in 37% to 59% of males arrested for violent crimes in U.S.cities.⁸ Among Finnish males, 40% of the risk for homicide is attributable to alcoholism.⁹ There are two types of alcoholism.¹⁰ Type 2 accounts for most of the violence associated with alcohol abuse. This type, transmitted from fathers to sons, develops early in life. Type 2 abusers frequently fight under the influence of alcohol and show features of antisocial personality disorder. Personality disorders, particularly the antisocial personality and the borderline personality disorders, are frequently manifested by violent behavior.

A proportion of violent acts occurring in the community are committed by persons diagnosed with major mental disorders such as schizophrenia or mood disorders. Although these disorders carry an elevated risk for violent crime,^9,11 only about 4% of the risk for violence self-reported by community residents in the United States is attributable to such disorders.² However, major mental disorders are frequently comorbid with substance abuse,¹² and this comorbidity further elevates the risk for violent behavior.^2,13 Patients with major mental disorders who stop taking their medication are at a higher risk for developing violent behavior than those who adhere to the treatment.¹⁴ The combination of substance abuse and nonadherence to treatment places patients at a particularly high risk for violence.¹⁵ Brain injuries, particularly those affecting the frontal ventromedial areas¹⁶ or the temporal lobes, are frequently associated with violent behavior. Dementia, mental retardation, and essentially any other disease or disorder affecting the brain may elicit impulsive violent behavior through cognitive impairment and a failure of inhibitory control.

Rearing environment has a powerful effect on behavior, including violence (Figure 1). At least one-third of victims of child abuse grow up to continue the pattern of inept, neglectful, or abusive rearing as parents.¹⁷ Many aspects of the rearing environment are determined by the parents' behavior; that behavior is of course not independent of the parents' genes (a factor not shown in Figure 1). Furthermore, the rearing environment is partly shaped by the child's behavior. Thus, genetic and environmental factors continuously interact in child development.

It is clear that maladaptive behaviors can be learned, but this is not the only mechanism of the intergenerational transfer of violence. Well before any teaching or role modeling takes place, the development of the brain may be affected by various factors in the prenatal and perinatal environment. Fetal exposure to alcohol may contribute to the development of violent behavior.^18,19 Early maternal rejection of the child interacts with obstetrical complications to predispose the individual to later (adult) violence.²⁰ Aggressive behavior is heritable,^21,22 and these pre- and perinatal events interact with genetic factors. Some of these complex relationships are depicted in Figure 1.

The clinical correlates of suicidal and violent behavior overlap: alcohol and drug abuse, personality disorders, schizophrenia, early life experiences, and various brain disorders predispose to both types of behavior.³

NEUROTRANSMITTERS, THEIR GENETIC CONTROL, AND VIOLENCE

Many neurotransmitters and hormones, including vasopressin,²³ steroids, opioids, and other substances, are involved in the modulation of aggressive behavior. Most of the current evidence strongly supports the roles of serotonin and catecholamines.

Serotonin (5-hydroxytryptamine; 5-HT) exerts inhibitory control over impulsive aggression. It is formed in the body by hydroxylation and decarboxylation of the essential amino acid tryptophan. The hydroxylation is the rate-limiting step in 5-HT synthesis; it is catalyzed by tryptophan hydroxylase (TPH). The gene for TPH has been cloned and mapped to the short arm of chromosome 11; two polymorphisms have been identified. The levels of a serotonin metabolite, 5-hydroxyindoleacetic acid (5-HIAA) in the cerebrospinal fluid (CSF) of impulsive offenders were associated with TPH genotype.²⁴ In general, these levels are believed to reflect presynaptic serotonergic activity in the brain. Reduced CSF 5-HIAA levels thus indicate a reduction in central serotonergic activity. At the synaptic level, reuptake is a mechanism for the termination of the action of serotonin. This reuptake is accomplished by a plasma membrane carrier called serotonin transporter (5HTT). The gene for 5HTT has been mapped to chromosome 17; a polymorphism has been described.²⁵ Thus, in summary, serotonin synthesis and activity regulation are under genetic control.

These indices of serotonergic transmission were examined in relation to violent and suicidal behavior. TPH genotype was associated with impulsive-aggressive behavior in male (but not female) patients who had personality disorders²⁶ as well as with suicide attempts in impulsive offenders²⁴ and in nonoffenders.²⁷ Low levels of CSF 5-HIAA have been reported in persons who attempted suicide (particularly by violent means)²⁸ and in persons with personality disorders who showed high levels of lifetime aggressiveness.²⁹ In these subjects, there was an association between aggressiveness and suicidal behavior.²⁹ This trivariate relationship between low levels of CSF 5-HIAA, impulsive aggression, and suicide has been replicated in various other populations.^3,4 As mentioned above, type 2 alcoholics frequently exhibit violent behavior; there is a common predisposition underlying the transmission of both alcoholism and violent behavior in families. Type 2 alcoholism is associated with a serotonergic deficit.^30–32 An association between low-activity 5HTT promoter genotype and early-onset alcoholism with habitual impulsive violent behavior has been observed.³³ Furthermore, a polymorphism of the 5-HT_1B receptor gene was linked to alcoholism with aggressive and impulsive behavior.³⁴ A nonhuman primate model confirmed that the serotonergic deficit, assessed by CSF 5-HIAA concentrations, is an antecedent (not a consequence) of alcohol consumption.³⁵

Neuroendocrine challenges offer another method of indirectly studying central serotonergic activity. A single dose of fenfluramine elevates the plasma levels of prolactin. This response is mediated by serotonergic action, and the prolactin elevation thus measures central serotonin activity. Prolactin response is reduced in aggressive subjects; this measure may be more sensitive than the CSF 5-HIAA.³⁶

These measures demonstrated a central serotonergic deficit associated with violence, but they could not locate the deficit to any area within the brain. Such localization has now become possible. Localized reductions of the 5HTT-specific binding were visualized in the living human brain.³⁷ I will return to these results in the section on brain imaging.

Thus, in summary, the association between impulsive violent behavior and central serotonin deficit has been demonstrated by using varied measures of serotonin function. The subjects in these studies were largely diagnosed with personality disorders and alcoholism. The role of serotonergic mechanisms in violent behavior accompanying other disorders is not clear; a small study has failed to detect low levels of CSF 5-HIAA in violent schizophrenic patients.³⁸

Norepinephrine systems also play a role in aggression. Similar to suicidal behavior, aggression is associated with increased noradrenergic activity. Stress-elicited noradrenergic activity is linked to irritable aggression in animals. The evidence for noradrenergic involvement in human aggression is indirect. Plasma levels of epinephrine and norepinephrine were related to experimentally induced hostile behavior in normal subjects.³⁹ Growth hormone response to clonidine, an alpha-2-adrenergic receptor agonist, was related to irritability (but not to assaultiveness) in normal subjects and in patients with personality disorders.⁴⁰ Furthermore, beta-adrenergic blocking agents have been used clinically to suppress violent behavior in patients with a variety of neuropsychiatric disorders.⁴¹

Two enzymes are important in the catabolism of catecholamines, including norepinephrine: monoamine oxidase (MAO) and catechol-O-methyltransferase (COMT). MAO exists in two forms, A and B. Both types are present in the brain; type B is also in the platelets where it can be assayed. Genes for both types are located at the X chromosome. Several lines of evidence link MAO to aggressive behavior. Low MAO activity was found in the platelets of violent offenders.⁴² In a large kindred, several males showed consistent impulsive violent behavior and mental retardation; each of these males had a point mutation in the MAO-A structural gene that resulted in a complete and selective deficiency of the activity of this enzyme.⁴³ Male “knockout” (genetically altered) mice lacking the MAO-A gene showed aggressive behavior.⁴⁴ Thus, low levels of MAO activity were associated with impulsive aggression.

COMT activity is governed by a common polymorphism that results in 3- to 4-fold variations in enzymatic activity. Two studies have demonstrated an association between the allele coding for the less active form of the enzyme and violent behavior in schizophrenic and schizoaffective patients.^45,46 This association seemed more expressed in males. These small association studies need to be replicated in larger samples of schizophrenic patients and extended to nonschizophrenic subjects. Male (but not female) knockout mice deficient in the COMT gene exhibited aggressive behavior.⁴⁷ Thus, in summary, high noradrenergic activity and low activity of the enzymes that catabolize catecholamines are associated with aggressive behavior. The roles of dopaminergic mechanisms in relation to alcoholism and personality traits are being studied; the results have not yet provided a generally accepted pattern of association with violence.

In the preceding paragraphs, I discussed various polymorphisms that contribute to the control of neurotransmission. These polymorphisms are genetically transmitted; this is the template function of the gene. This function is not amenable to any changes due to environmental effects except for mutations. However, the transcriptional function that determines the gene expression (i.e., the manufacture of specific proteins) does depend on environmental and social factors.⁴⁸ Gene expression can be modified, for example, by the rearing environment: CSF 5-HIAA in monkeys varies depending on whether they were reared by their mothers or by peers⁴⁹ (see Figure 1).

GENDER AND VIOLENCE

Gender is a very robust predictor of violent behavior⁴ and of suicide.³ In the United States, male suspects account for 85% of arrests for violent crimes.¹ Victimization surveys as well as self-reports by adults living in the community² have generally supported the predominance of males among perpetrators of violence. However, this gender difference is reduced among community residents with major mental disorders and those with substance use disorders,² and it disappears among hospitalized psychiatric patients.⁴

The gender difference in aggressiveness develops in preschool years, and it is fully expressed by puberty. The difference is partly due to societal causes, including child-rearing practices. Numerous lines of evidence suggest biological origins of the gender difference. As noted above, the TPH, MAO-A, and COMT genotypes show associations with violent behavior that are largely limited to males. Males are more likely than females to develop alcoholism, particularly type 2 alcoholism that is associated with aggression. Elevated circulating testosterone level may be associated with aggression in young males,⁵⁰ and perhaps even in young females,⁵¹ but these findings have not been consistently replicated; furthermore, it is not clear whether the hormonal level is an antecedent or a consequence of violent behavior.⁵²

BRAIN STRUCTURE AND FUNCTION

The orbitomedial prefrontal cortex is involved in controlling and inhibiting impulsive actions, and lesions to this area may result in disinhibited aggressive^4,16 or suicidal behaviors.³ Furthermore, literature on aggression points to a dysfunction of parts of the limbic system, particularly the amygdala and the hippocampus, the two limbic structures within the temporal lobe.⁵³ There is some evidence that the frontal and temporal abnormalities associated with violence may be more expressed in the dominant hemisphere. Until recently, electroencephalography was the main method of studying the function of these systems in humans; this approach was useful in confirming frontotemporal abnormalities in violent individuals (particularly in impulsive violence), but the information was relatively nonspecific in terms of the nature of the dysfunction and its subcortical localization.^54,55 More recent imaging methods such as positron emission tomography (PET) and single-photon emission computed tomography (SPECT) are providing more specific information. Glucose utilization and blood flow measurements using PET showed abnormality in the left temporal lobe and in the frontal lobes of violent psychiatric patients.⁵⁶ Mentally ill murderers showed selective reductions in prefrontal glucose utilization during a continuous performance task.⁵⁷ A reanalysis of the data obtained in this study showed that the murderers who had no history of early psychosocial deprivation (e.g., no childhood abuse or family neglect) had lower prefrontal glucose metabolism than murderers with early psychosocial deprivation and a group of normal controls.⁵⁸ Thus, these results suggest the existence of two paths toward the development of violent behavior: abnormal neurobiology (prefrontal cortical dysfunction), or abnormal rearing environment. Another reanalysis of these data⁵⁷ determined that the impulsive (but not the predatory) offenders had lower prefrontal glucose metabolism in comparison with the control subjects.⁷ Thus, prefrontal cortical functioning that controls aggressive behavior was impaired specifically in persons who exhibit impulsive (but not premeditated) violence.

The PET results discussed have revealed brain dysfunctions that were localized, but not specifically linked to any neurotransmitter system. A recent SPECT study reported such specific findings. The 5HTT binding in the midbrain and in the occipital and the frontal mesial cortex of alcoholic impulsive violent offenders was lower than that in healthy control subjects or in nonviolent alcoholics.³⁷ Interestingly, autoradiographic studies of suicide victims' postmortem brain tissue have also revealed serotonin receptor abnormalities in the ventral prefrontal cortex.³ The recent PET and SPECT results discussed above will have to be replicated.

If these studies are correct, what are the origins of the brain dysfunctions they reveal? They may be genetic, or due to noxious environmental influences disturbing prenatal neurodevelopment, or related to perinatal complications, or an interaction among these factors. Some structural changes, such as a reduction of the temporal lobe volume, may be caused by child abuse; this effect could be mediated by chronic elevations of glucocorticoids and catecholamines associated with the stress of being maltreated.⁵⁹ Similarly, a quantitative EEG study provided preliminary evidence for aberrant cortical development in abused children.⁶⁰

PREVENTION AND TREATMENT OF VIOLENCE

Improvements in prenatal and perinatal care and prevention of head injuries may reduce the level of violence in the community. Since substance use disorders play such an important role in violence, it seems that recidivistic violent crime could be substantially reduced if the treatment of substance use disorders were routinely available to prisoners. In the United States, the availability is inadequate. Facilities that care for schizophrenic and manic patients need to focus on the diagnosis and treatment of the frequently comorbid substance use disorders. Adherence to treatment must be strongly encouraged and monitored in patients with major mental disorders who have a history of recidivistic violence after they discontinue their medication.

Pharmacological treatment of violent behavior in patients with mental disorders is somewhat similar to the treatment of patients with suicidal ideation. Selective serotonin reuptake inhibitors (SSRIs) that are routinely used to treat depression were also effective against impulsive aggression in patients with personality disorders who were not suffering from major depression.⁶¹ Clozapine, an atypical antipsychotic medication, is more effective than other antipsychotics in reducing both aggressive⁴¹ and suicidal³ behaviors in schizophrenia. Pharmacological reduction of violent behavior in persons without mental disorders might be possible, but it could raise ethical issues that have not been addressed. Lithium, a medication that appears to reduce suicide risk for bipolar patients,³ also reduced impulsive aggressive behavior in prisoners who were not psychotic;⁶² both effects could be mediated by an enhancement of serotonergic activity. The administration of an SSRI for 4 weeks to persons without mental disorders has reduced their feelings of hostility.⁶³ Pharmacological treatments of aggression have been recently reviewed in detail elsewhere.^41,64,65

FUTURE DIRECTIONS

Phenotypic heterogeneity of violent behaviors is a principal problem of this research area. Efforts at subtyping of violence (e.g., impulsive versus premeditated violence) are in progress, but much remains to be done. Most of the neurobiological tools I discussed have separated, one tool at a time, a group of violent subjects from a group of control subjects. Very rarely has a single tool been used to define subgroups of violent patients; using more than one tool for such grouping is even rarer. But even violent behaviors that are phenotypically identical may be heterogeneous from the standpoint of underlying neurobiology.

A fundamental challenge is to translate the recognition of this heterogeneity into research, prevention, and treatment. This requires using the available tools of molecular genetics, neuropharmacology, brain imaging, and psychopharmacology in the same subjects. In this way, it may, for example, become possible to identify patients with specific serotonergic or noradrenergic deficits that could be corrected pharmacologically, or, in the future, perhaps using gene therapy. Furthermore, such a multidisciplinary approach would enable a search for protective factors that might explain, for example, why some patients with very low CSF 5-HIAA do not become violent. Knowledge of such protective factors would be useful in developing strategies for prevention and treatment.

Testing the efficacy of antiaggressive treatments will require substantial modifications of the classic trial designs that have been used in psychopharmacology for the past three decades. The problems to be addressed include the need for precise definition of violent behavior, the difficulty of measuring outcome because of the relative rarity of violent incidents, bias in the selection of patients for study, inadequate and inappropriate control groups, and inattention to comorbidities and concomitant medications in analyzing results.⁶⁶

Neurobiological and environmental (e.g., social) factors continuously interact and influence each other. For example, obstetrical complications and maternal rejection interact to raise the propensity for violence, and they may influence each other (e.g., a child with brain dysfunction resulting from obstetrical complications may behave in a way that provokes maternal rejection). To understand the neurobiology of violence, we need to study it in the context of such biosocial interactions.

CONCLUSIONS

The past five years have brought significant progress in our understanding of the neurobiology of violence. The field has been enriched by numerous contributions in the areas of molecular genetics and brain imaging. With deeper understanding of the underlying neurobiology, commonalities between violent and suicidal behaviors can be seen more clearly now.

Up until recently, the assessment of biosocial interactions that result in violent behavior was largely limited to acknowledgments of their existence; we have now progressed to formal statistical demonstrations of these interactions. The “nature or nurture” controversy inherited from Francis Galton has been an underlying philosophical basis of numerous political problems that have plagued the field of violence research during the past decade.⁶⁷ The recent studies of biosocial interactions have rendered the controversy of nature and nurture moot; nature is nurture.